The plant list includes a further 8 scientific plant names of infraspecific rank for the genus dioclea. Effect of leguminous lectins on the growth of rhizobium. Briefly, the seeds were ground into a fine powder and stirred with 0. Isolation and characterization of novel lectins from.
Diocleinae lectins are a group of proteins with conserved. See status, confidence level, source for definitions. Structurally, dvl appears in tetrameric form in neutral ph and dimeric form in more acidic ph. Dioclea is a genus of flowering plants in the pea family, fabaceae, that is native to the americas. Dioclea violacea lectin ameliorates oxidative stress.
Dioclea violacea lectin ameliorates oxidative stress and renal. The seeds of these legumes are buoyant drift seeds, and are dispersed by rivers. Cancer remains a global health challenge, with high morbidity and mortality, despite the recent advances in diagnosis and treatment. The record derives from ildis data supplied on 20100714 which reports it as an accepted name record 16815. Differences in structure of lectins were found to be re. Read structural analysis of a dioclea sclerocarpa lectin. Our data demonstrate that dvl administration in ir animals increases glomerular filtration rate, renal plasma and blood flow, while, at the same time, it diminishes renal vascular resistance. Pdf dioclea violacea lectin ameliorates oxidative stress and renal. The integrated taxonomic information system itis provides authoritative taxonomic information on dioclea reflexa, as well as other plants, animals, fungi, and microbes of north america and the world. Pdf isolation and partial characterization of a lectin from. It is invading natural habitats where it is outcompeting native species. A bulbous, stemless perennial typically growing 48 tall in which the longstemmed leaves and longer, leafless flower stalks rise directly from the bulb. Plantderived lectins as potential cancer therapeutics and.
Molecules free fulltext effect of leguminous lectins on. Lectins from cratylia floribunda cfl, dioclea violacea dvl, and canavalia gladiata cgl were purified as previously reported 1921. The galactose specific lectin leca mediates biofilm formation in the opportunistic pathogen p. Acute kidney injury aki is characterized by rapid and potentially reversible decline in renal function. Promastigote attachment to sand fly midgut epithelium is essential to avoid being excreted together with the digested blood meal. Plants mostly herbs rarely shrubs, all leaves basal, simple, stipulate. International journal of biological macromolecules 120, 566577, 2018. The generality of this interaction was tested in a. We do not intend the plant list to be complete for names of infraspecific rank. In this study lectins purified from the seeds of dioclea violacea dvl and dioclea. Dioclea kunth show all show tabs dioclea general information. Lectin was extracted from the seeds of dioclea violacea dvl by affinity chromatography as previously described. Concanavalin a as an inducer of human lymphocyte mitogenic. Fluorescence micrography was employed to visualize the ability of fitc.
Dioclea or diocleia, and also dioklea or diokleia may refer to. Oxalis violacea violet wood sorrel pfaf plant database. Oxalis violacea, commonly called violet wood sorrel, is a common missouri native wildflower which occurs statewide in dryish, acidic soils on glades, rocky open woods, fields and prairies. In vitro inhibition of streptococci binding to enamel. Jun 18, 2014 this page was last edited on 3 september 2017, at 07. Access is allocated within two business days of purchase and lasts one year. The lectin extracted from the seed of dioclea violacea, dvl, was purified for the first time in 1996 by moreira et al. In this study, the dglucose dmannosebinding lectins from seeds of dioclea megacarpa, d. All structured data from the file and property namespaces is available under the creative commons cc0 license. Protein science, the flagship journal of the protein society, serves an international forum for publishing original reports on all scientific aspects of protein molecules. The sdspolyacrylamide gel electrophoresis patterns of the subunit structures of the lectins resemble cona and d. Dec 15, 2015 dioclea violacea lectin ameliorates oxidative stress and renal dysfunction in an experimental model of acute kidney injury. Less potency and efficacy was observed forcanavalia maritima, dioclea guianensis.
Histamine release induced by glucose mannosespecific. Susceptibility and refractoriness to leishmania depend on the outcome of multiple interactions that take place within the sand fly gut. These results indicate that dvl can be considered a new therapeutic approach for akiinduced kidney injury. Dioclea in phrygia, an ancient city and former bishopric in phrygia asia minor. Dioclea virgata wikispecies wikispecies, free species. Ch tshape interaction with histidine explains binding.
The international legume database and information service or ildis tropicos link. Antiglioma properties of dvl, a lectin purified from dioclea violacea apm nascimento, jl knaut, dk rieger, iav wolin, ia heinrich, j mann. Dioclea violacea lectin ameliorates oxidative stress and renal dysfunction in an experimental model of acute kidney injury. Plants have escaped from cultivation and, in cuba, the species is listed as invasive and as one of the more noxious invasive plants.
Contents the international journal of biochemistry cell biology. Manual adjustments in coot were made after each refinement step and an x man. This page was last edited on 3 september 2017, at 07. Pdf isolation and partial characterization of a lectin from seeds of.
Briefly, the lectins were extracted from airdried ground seeds collected in fortalezace, brazil, and defatted with nhexane. Protein science aims to unify this field by cutting across. Dvl is a legume lectin with affinity for glucosemannose, and it belongs to the subtribe diocleinae, similar to cona. Leishmaniasis is a vectorborne disease transmitted by phlebotomine sand fly. The effects of the lectin of dioclea violacea dvl, purified by moreira et al. Dioclea state, a medieval serb state of duklja, in southeastern part of modern montenegro dioclea in praevalitana, an ancient roman and byzantine city in the province of praevalitana, near modern podgorica in montenegro. Antiglioma properties of dvl, a lectin purified from. Pdf dioclea violacea martius, ex bentham, contain a lectin that could. Determinants of quaternary association in legume lectins. All these activities were reversed by binding the lectin to glucose or. Two lectins were isolated from canavalia ensiformis and dioclea grandiflora seeds. Berman, rutgers board of governors professor of chemistry and. This lectin exhibits various pharmacological properties.
Flavia ps freitas physiological sciences graduate program, health sciences center, federal university of espirito santo vitoria, es, brazil. Pdf isolation and partial characterization of a lectin. Lectins, a group of highly diverse proteins of nonimmune origin with carbohydratebinding abilities, have been detected in. Acute kidney injury, ischemia reperfusion, renal function, plant lectin, dioclea violacea. Contents the international journal of biochemistry cell. Antinociceptive activity of lectins from diocleinae seeds.
The lectins are from canavalia brasiliensis, canavalia bonariensis, cratylia floribunda, dioclea rostrata, dioclea virgata, dioclea violacea, and dioclea guianensis. The percentage of the lectins antinociception varied from 61% for canavalia grandiflora congf to 20% for dioclea violacea. Less potency and efficacy was observed forcanavalia maritima, dioclea guianensis, anddioclea violacea while very low. Ipni nomenclature info for dioclea reflexa nomenclatural information about dioclea reflexa is provided by the international plant names index ipni. The lectins used in this work were extracted from canavalia ensiformis, canavalia brasiliensis, dioclea violacea, dioclea grandiflora, cratylia floribunda and vatairea macrocarpa. Text is available under the creative commons attributionsharealike license. The journal publishes papers by leading scientists from all over the world that report on advances in the understanding of proteins in the broadest sense. Diocleinae lectins administered per oral route in mice inhibited the abdominal constrictions induced by acetic acid. Thecanavalia brasiliensis, dioclea rostrata, anddioclea virgata lectins induced histamine release in rat peritoneal mast cells similar to concanavalin a. Crystal structure of dioclea violacea lectin and a comparative study. Ontario, canada south to georgia and westward to texas, oklahoma, kansas and nebraska known invasiveness. Caesalpinia violacea is a fastgrowing tree with the capability to grow and establish in poor clay, stony and sandy soils. Diocleinae lectins are a group of proteins with conserved binding. The plant list includes 95 scientific plant names of species rank for the genus dioclea.
Dioclea violacea lectin dvl is a protein that recognizes and binds primarily to manglc saccharides present in cell surface glycans 17. Briefly, the lectins were extracted from airdried ground seeds collected in fortalezace, brazil, and defatted with n. Crystal structure of dioclea violacea lectin and a comparative study of vasorelaxant properties with dioclea rostrata lectin. Pdf acute kidney injury aki is characterized by rapid and potentially reversible decline in renal. The histamine releasing properties of glucose mannosespecific lectins isolated from brazilian beans was examined. Lectin from the seeds of dioclea sclerocarpa dsl was purified in a single step by affinity chromatography on a sephadex g50 column. Lectins, a group of highly diverse proteins of nonimmune origin with carbohydratebinding. Antiglioma properties of dvl, a lectin purified from dioclea. All of the lectins exhibited specificity for man and glc residues, but much higher affinities for the branched chain trimannoside, 3,6di o. Files are available under licenses specified on their description page. Crystal structure of dioclea violacea lectin and a. The sdspolyacrylamide gel electrophoresis patterns of the subunit.
Dioclea violacea lectin ameliorates oxidative stress and. Jan 01, 2016 read structural analysis of a dioclea sclerocarpa lectin. Thermodynamic binding studies of lectins from the diocleinae. It is in flower from may to august, and the seeds ripen from july to september. Please note that this is a trial program and may end. J f m a m j j a s o n d a tuftforming bulbous plant with straplike greygreen leaves that are strongly garlic scented when bruised. Ch tshape interaction with histidine explains binding of. Enjoy unrestricted access to all content and features, including. Violet lespedeza lespedeza violacea propagation protocol. The structural basis of the ph dependency of the dimertetramer transition exhibited by brindas type ii diocleinae lectins was investigated by equilibrium sedimentation and x.
Study on the vasorelaxant properties of dioclea lectins, international journal of biological macromolecules on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Gel filtration produced a fraction corresponding to con a or d. Discover lifes page about the biology, natural history, ecology, identification and distribution of dioclea wilsonii wilsons clusterpea discover life. A lectin from dioclea violacea interacts with midgut. Dioclea violacea lectin ameliorates oxidative stress and renal dysfunction in an experimental model of acute kidney injury flavia ps freitas, 1 marcella l porto, 1 camilla p tranhago, 1 rogerio piontkowski, 2 emilio c miguel, 3 thaiz bar miguel, 3 jorge l martins, 4 kyria s nascimento, 3 camille m balarini, 5 benildo s cavada, 3. Lectin from dioclea violacea induces autophagy in u87 glioma cells ana paula m. Crystallization kits were used to obtain protein crystals and the better condition was 4m sodium formate. The primary sequence, as determined by tandem mass spectrometry, revealed a protein with 237 amino acids and 81% of identity with cona. The generality of this interaction was tested in a diverse. In the present study, we evaluated the effects of dioclea violacea lectin administered prior to ischemiareperfusion irinduced acute kidney injury aki. The lectins included those from canavalia brasiliensis, cratylia floribunda, dioclea rostrata, dioclea virgata, dioclea violacea, and dioclea guianensis. The species is hermaphrodite has both male and female organs and is pollinated by insects.
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